日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响

doi:10.11843/j.issn.0366-6964.2013.12.008

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 1913-1918.doi: 10.11843/j.issn.0366-6964.2013.12.008

日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响

王军^{1, 2}，孙蕾¹，周海柱²，张涛³，徐晶²，娄玉杰^2*

（1.辽宁医学院畜牧兽医学院，锦州 121001； 2.吉林农业大学动物科技学院，长春 130118；3 .雪龙黑牛股份有限公司，大连 116031）

收稿日期:2013-07-08 出版日期:2013-12-23 发布日期:2013-12-23
通讯作者: 娄玉杰，E-mail: louyujiejlau@126.com
作者简介:王军(1979-)，男，吉林敦化人，博士，副教授，主要从事动物繁殖调控研究， E-mail: junwang2004@126.com
基金资助:
辽宁省教育厅项目（L2010255）；吉林省人力资源和社会保障厅项目（2010273）

Effect of Low Dietary Nutrition on Hypothalamic Expression of KiSS-1/GPR54 System and Relative Hormones Secretion in Ewes

WANG Jun^{1, 2}，SUN Lei¹，ZHOU Hai-zhu²，ZHANG Tao³，XU Jing²，LOU Yu-jie^2*

（1.College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, China； 2.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China； 3.Snowdragon Beef Co., Ltd, Dalian 116031, China）

Received:2013-07-08 Online:2013-12-23 Published:2013-12-23

摘要/Abstract

摘要：

本研究通过分析日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响，明确KiSS-1/GPR54系统在日粮营养调控母羊初情期启动中的作用。选择12只体重为16 kg左右的3月龄雌性小尾寒羊，随机分为2组（n=6），分别给予标准营养和低营养（对照组采食量的60%），饲喂30 d后，采集下丘脑和血液，利用实时荧光定量PCR、免疫组化、ELISA和放免技术测定下丘脑KiSS-1/GPR54系统表达量及LH、胰岛素和Leptin浓度。结果表明，日粮低营养可显著降低母羊下丘脑弓状核KiSS-1基因和蛋白的表达量（P＜0.01），但对GPR54基因表达量并无影响（P＞0.05）；日粮低营养可显著降低母羊血清中LH和胰岛素（P＜0.05）浓度，极显著降低Leptin浓度（P＜0.01）。结果提示，下丘脑KiSS-1/GPR54系统可通过降低KiSS-1表达来参与日粮低营养对初情期启动的调控。

Abstract:

The present study was conducted to confirm the role of KiSS-1/GPR54 system in nutrition regulation of puberty onset by analyzing effect of low dietary nutrition on hypothalamic expression of KiSS-1/GPR54 system and relative hormones secretion in ewes.Twelve female Small-tailed Han sheep (native breed), around 16 kg body weigh and three months old, were randomly divided into control and feed restriction group (n=6). Control group was fed with normal nutrition diet and the feed restriction group fed with low nutrition diet (60% of food intake of control group). Thirty days latter, ewes were killed, and hypothalamus and blood were collected for determining hypothalamic expression of KiSS-1/GPR54 system and secretion of LH, insulin and Leptin in ewes. The results showed that low nutrition could significantly decrease expression of KiSS-1 gene and protein in arcuate nucleus of hypothalamus of ewes（P＜0.01）, however, there was no significant difference on the expression of GPR54 between two groups（P＞0.05）.Low nutrition also led to decreasing of LH, insulin（P＜0.05） and Leptin（P＜0.01）in serum of ewes. These results indicated that hypothalamic KiSS-1/GPR54 system may involve in low nutrition regulation of puberty onset by decreasing the expression of KiSS-1 in ewes.

中图分类号:

S826
S814.1

王军，孙蕾，周海柱，张涛，徐晶，娄玉杰. 日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响[J]. 畜牧兽医学报, 2013, 44(12): 1913-1918.

WANG Jun，SUN Lei，ZHOU Hai-zhu，ZHANG Tao，XU Jing，LOU Yu-jie. Effect of Low Dietary Nutrition on Hypothalamic Expression of KiSS-1/GPR54 System and Relative Hormones Secretion in Ewes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1913-1918.

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日粮低营养对母羊下丘脑KiSS-1/GPR54系统表达及相关激素分泌的影响

Effect of Low Dietary Nutrition on Hypothalamic Expression of KiSS-1/GPR54 System and Relative Hormones Secretion in Ewes

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